The volume of mobile phase passing through the column in unit time. The flow rate is usually measured at column outlet, at ambient pressure
(
) and temperature
(
, in
); this value is indicated with the symbol
. If a water-containing flowmeter was used for the measurement (e.g. the so-called
soap bubble flowmeter) then
must be corrected to dry gas conditions in order to obtain the mobile phase flow rate at ambient temperature
(
):
where
is the
partial pressure of water vapour at ambient temperature. In order to specify chromatographic conditions in
column chromatography, the flow-rate (mobile phase flow rate at column temperature,
) must be expressed at
(
kelvin), the column temperature:
Source:
PAC, 1993, 65, 819
(Nomenclature for chromatography (IUPAC Recommendations 1993))
on page 839
Cite as:
IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"). Compiled by A. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997). XML on-line corrected version: http://goldbook.iupac.org (2006-) created by M. Nic, J. Jirat, B. Kosata; updates compiled by A. Jenkins. ISBN 0-9678550-9-8.
doi:10.1351/goldbook.
