A ligand-binding
assay that uses a specific
antigen or
antibody, capable of binding to the analyte, to identify and quantify substances. The
antibody can be linked to a radiosotope (
radioimmunoassay,
RIA), or to an
enzyme which catalyses an easily monitored reaction (enzyme-linked immunosorbent
assay,
ELISA), or to a highly fluorescent compound by which the location of an
antigen can be visualized (immunofluorescene).
Source:
PAC, 1992, 64, 143
(Glossary for chemists of terms used in biotechnology (IUPAC Recommendations 1992))
on page 157
PAC, 1994, 66, 2513
(Nomenclature for radioanalytical chemistry (IUPAC Recommendations 1994))
on page 2520
Cite as:
IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"). Compiled by A. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997). XML on-line corrected version: http://goldbook.iupac.org (2006-) created by M. Nic, J. Jirat, B. Kosata; updates compiled by A. Jenkins. ISBN 0-9678550-9-8.
doi:10.1351/goldbook.
