IUPAC > Gold Book > alphabetical index > P > photoaffinity labelling
icon

IndexesDownload


photoaffinity labelling

Acronym: PAL

Technique by which a photochemically reactive molecular entity, specifically associated with a biomolecule, is photo-excited in order to covalently attach a label to the biomolecule, usually via intermediates. Identification of the resulting photo-crosslinked product provides structural information on the protein′s binding site.
Notes:
  1. Technique widely used for identifying in general ligand-receptor interactions in biological macromolecules and in particular the binding site within proteins.
  2. Typical examples are nitrenes, carbenes, ketone excited states, cations and radicals.
Source:
PAC, 2007, 79, 293 (Glossary of terms used in photochemistry, 3rd edition (IUPAC Recommendations 2006)) on page 383
Interactive Link Maps
First LevelSecond LevelThird Level
GraphGraphGraph
Cite as:
IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"). Compiled by A. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997). XML on-line corrected version: http://goldbook.iupac.org (2006-) created by M. Nic, J. Jirat, B. Kosata; updates compiled by A. Jenkins. ISBN 0-9678550-9-8. doi:10.1351/goldbook.
Last update: 2014-02-24; version: 2.3.3.
DOI of this term: doi:10.1351/goldbook.P04578.
Original PDF version: http://www.iupac.org/goldbook/P04578.pdf. The PDF version is out of date and is provided for reference purposes only. For some entries, the PDF version may be unavailable.
Current PDF version | Version for print | History of this term
picture