Technique by which a photochemically reactive molecular entity, specifically
associated with a biomolecule, is photo-excited in order to covalently attach a
to the biomolecule, usually via intermediates. Identification of the resulting
photo-crosslinked product provides structural information on the protein′s binding site
- Technique widely used for identifying in general ligand-receptor interactions in
biological macromolecules and in particular the binding site within proteins.
- Typical examples are nitrenes, carbenes, ketone excited states, cations and radicals.
PAC, 2007, 79, 293
(Glossary of terms used in photochemistry, 3rd edition (IUPAC Recommendations 2006))
on page 383
IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"). Compiled by A. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997). XML on-line corrected version: http://goldbook.iupac.org (2006-) created by M. Nic, J. Jirat, B. Kosata; updates compiled by A. Jenkins. ISBN 0-9678550-9-8. doi:10.1351/goldbook