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difference absorption spectroscopy

A highly concentrated analyte in an analytical sample can be determined with better precision by replacing the blank (reference) cell by one containing a solution of the analyte or other absorber of known concentration; this is known as difference absorption spectroscopy. Difference spectra can also be obtained by computer or other subtraction methods.
Source:
PAC, 1988, 60, 1449 (Nomenclature, symbols, units and their usage in spectrochemical analysis - VII. Molecular absorption spectroscopy, ultraviolet and visible (UV/VIS) (Recommendations 1988)) on page 1455
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IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"). Compiled by A. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997). XML on-line corrected version: http://goldbook.iupac.org (2006-) created by M. Nic, J. Jirat, B. Kosata; updates compiled by A. Jenkins. ISBN 0-9678550-9-8. doi:10.1351/goldbook.
Last update: 2014-02-24; version: 2.3.3.
DOI of this term: doi:10.1351/goldbook.D01701.
Original PDF version: http://www.iupac.org/goldbook/D01701.pdf. The PDF version is out of date and is provided for reference purposes only. For some entries, the PDF version may be unavailable.
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