Goldbook title
IUPAC > Gold Book > alphabetical index > G > Gram stain
Gold G Icon
Indexes Download

Gram stain

A set of two stains that are used to stain (crystal violet and iodine) and counterstain (fuchsin) bacteria. Gram-negative bacteria are completely decolorized after treating the stained bacteria with ethanol or acetone and can be visualized by the counterstain. They have a complex cell wall in which the peptidoglycan layer is covered by lipopolysaccharides in an outer membrane structure. Gram-positive bacteria resist decolorization by ethanol or acetone, they retain the initial Gram stain. They have a cell wall consisting predominantly of peptidoglycan not overlayed by an outer membrane.
PAC, 1992, 64, 143 (Glossary for chemists of terms used in biotechnology (IUPAC Recommendations 1992)) on page 155
Interactive Link Maps
First Level Second Level Third Level
Cite as:
IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"). Compiled by A. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997). XML on-line corrected version: (2006-) created by M. Nic, J. Jirat, B. Kosata; updates compiled by A. Jenkins. ISBN 0-9678550-9-8.
Last update: 2014-02-24; version: 2.3.3.
DOI of this term:
Original PDF version: The PDF version is out of date and is provided for reference purposes only. For some entries, the PDF version may be unavailable.
Current PDF version | Version for print | History of this term