1. of RNA: The procedure by which introns are removed from eukaryotic precursor mRNA molecules and adjacent exon sequences are joined together (spliced).
  2. of DNA: Manipulation for joining together double-stranded DNA fragments with protruding single-stranded 'sticky ends' by means of ligases.
PAC, 1992, 64, 143 (Glossary for chemists of terms used in biotechnology (IUPAC Recommendations 1992)) on page 166