A type of @A00307@ where the @C01281@ to be determined is amplified directly and it is this @C01281@ which is finally measured.
Source: PAC, 1982, 54, 2553. (Recommendations on use of the term amplification reactions) on page 2555 [Terms] [Paper]
Cite as: IUPAC. Compendium of Chemical Terminology, 2nd ed. (the "Gold Book"). Compiled by A. D. McNaught and A. Wilkinson. Blackwell Scientific Publications, Oxford (1997). Online version (2019-) created by S. J. Chalk. ISBN 0-9678550-9-8. https://doi.org/10.1351/goldbook.