frequency-domain fluorometry
Technique that permits @[email protected] of the parameters characterizing a @[email protected] decay. Instead of using an exciting @[email protected], UV, or @[email protected] radiation pulse (see @[email protected]), the sample is excited by sinusoidally modulated radiation at high @[email protected] The fluoresence response is sinusoidally modulated at the same frequency as, but delayed in phase and partially demodulated with respect to the excitation.
  1. The modulation ratio is defined as the ratio of the modulation depth (AC/DC ratio) of the @[email protected] and the modulation depth of the excitation. The phase shift and the modulation ratio characterize the harmonic response of the system. These parameters are measured as a function of the modulation frequency. No deconvolution is necessary because the data are directly analysed in the frequency domain.
  2. Phase and modulation measurements can be done by using either a @[email protected] (or a @[email protected]) and an optical modulator (in general a Pockel cell) or the harmonic content of a pulsed @[email protected]
PAC, 2007, 79, 293. (Glossary of terms used in photochemistry, 3rd edition (IUPAC Recommendations 2006)) on page 346 [Terms] [Paper]