frequency-domain fluorometry

https://doi.org/10.1351/goldbook.FT07384
Technique that permits @[email protected] of the parameters characterizing a @[email protected] decay. Instead of using an exciting @[email protected], UV, or @[email protected] radiation pulse (see @[email protected]), the sample is excited by sinusoidally modulated radiation at high @[email protected] The fluoresence response is sinusoidally modulated at the same frequency as, but delayed in phase and partially demodulated with respect to the excitation.
Notes:
  1. The modulation ratio is defined as the ratio of the modulation depth (AC/DC ratio) of the @[email protected] and the modulation depth of the excitation. The phase shift and the modulation ratio characterize the harmonic response of the system. These parameters are measured as a function of the modulation frequency. No deconvolution is necessary because the data are directly analysed in the frequency domain.
  2. Phase and modulation measurements can be done by using either a @[email protected] (or a @[email protected]) and an optical modulator (in general a Pockel cell) or the harmonic content of a pulsed @[email protected]
Source:
PAC, 2007, 79, 293. (Glossary of terms used in photochemistry, 3rd edition (IUPAC Recommendations 2006)) on page 346 [Terms] [Paper]