## flow rate

in chromatography
https://doi.org/10.1351/goldbook.F02439
The volume of mobile phase passing through the column in unit time. The flow rate is usually measured at column outlet, at ambient pressure ($$p_{\text{a}}$$) and temperature ($$T_{\text{a}}$$, in $$\text{K}$$); this value is indicated with the symbol $$F$$. If a water-containing flowmeter was used for the measurement (e.g. the so-called @[email protected] bubble flowmeter) then $$F$$ must be corrected to dry gas conditions in order to obtain the mobile phase flow rate at ambient temperature ($$F_{\text{a}}$$): $F_{\text{a}}=F\ (1- \frac{p_{\text{w}}}{p_{\text{a}}})$ where $$p_{\text{w}}$$ is the @[email protected] of water vapour at ambient temperature. In order to specify chromatographic conditions in @[email protected], the flow-rate (mobile phase flow rate at column temperature, $$F_{\text{c}}$$) must be expressed at $$T_{\text{c}}$$ (@[email protected]), the column temperature: $F_{\text{c}}=F_{\text{a}}\ \frac{T_{\text{c}}}{T_{\text{a}}}$
Source:
PAC, 1993, 65, 819. (Nomenclature for chromatography (IUPAC Recommendations 1993)) on page 839 [Terms] [Paper]