https://doi.org/10.1351/goldbook.G02681
A set of two stains that are used to stain (crystal violet and iodine) and counterstain (fuchsin) bacteria. @[email protected] bacteria are completely decolorized after treating the stained bacteria with ethanol or acetone and can be visualized by the counterstain. They have a complex cell wall in which the @[email protected] layer is covered by @[email protected] in an outer @[email protected] structure. @[email protected] bacteria resist decolorization by ethanol or acetone, they retain the initial @[email protected] stain. They have a cell wall consisting predominantly of @[email protected] not overlayed by an outer @[email protected]